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It is well-known that virgin queens produce fewer pheromones than mated queens, which can cause reluctance from the colony to accept them. To improve the chances of successful introduction, the following factors should be considered:
Introduce virgin queens promptly into the nuclei. The longer they wait, the lower the acceptance rate becomes.
A common practice is to immerse the virgin queen in liquid honey before releasing her into the mating hive. This allows the bees to gradually encounter her pheromones as they clean her, easing the acceptance process.
These methods help to ensure that the virgin queen is more likely to be accepted and integrated smoothly into the colony.



